Selectively Modulates Plasma Coagulation Protein Synthesis by the Perfused Rat Liver

The combined effects of orchiectomy and estrogen administration on synthesis of selected hepatic secretory proteins-antithrombin (AT) III, plasminogen, fibrinogen, factor II (prothrombin). factor VII. fibronectin. and albuminwere studied using the isolated rat liver perfused in vitro for ten hours. Male rat liver donors underwent orchiectomy under ether anesthesia and then received 5.0 mg of diethylstilbestrol (DES) by subcutaneous pellet implantation or a placebo pellet; 14 days later the livers were extracted and perfused in vitro for ten hours. In DES experiments, 1 .0 mg of DES was also added directly to the liver perfusate at the outset. Pretreatment with DES resulted in significant increases in cumulative synthesis of factors II (65%) and VII (76%) and significant reductions in cumulative synthesis of both antithrombin Ill (20%; P = .03) and plasminogen (27%; p < .01 ) compared to control perfusions. but synthesis of fibrinogen, fibronectin, and albumin was not significantly affected by addition of the hormone. Plasma samples collected from rat liver